Norovirus Internal Amplification Control

Description: RNA prepared using following steps:
1. Plasmid pnJVIAC containing fragment of Norovirus DNA was linearised with SalI restriction endonucleases and purified;
2. RNA was transcribed using T7 RNA polymerase transcription system;
3. Template DNA was removed from preparation during incubation with RNase-free Dnase;
4. RNA was purified with LiCl precipitation followed by multiple phenol/chlorophorm extractions.
5. Preparation was concentrated by precipitation with ethanol and dissolving in minimal volume of Milli-Q/18.2 Mom – quality water.

Pack Size: 50 µl

RNA Concentration: 1.0 mg/ml

Quality Control: denaturing electrophoresis revealed single band.

Shipping: blue-ice or dry ice, below 0ºC.

Storage: store at -70ºC for at least 6 months.

For research and in vitro use only. Not for drug, household or other uses.

Enquire on pnJIAC nucleotide sequence

Enquire on NOROIAC RNA sequence