Molecular Biology Grade Nanodiamonds

Description

References/Applications

Characteristics, properties

Ordering information

 

 Description

Yorkshire Bioscience offers modified Nanodiamonds (Molecular Biology Grade) with unique properties for physical, chemical and biology applications. Our first two products - NanoD1 and NanoD2 - are high purity homogeneous state nanodiamonds treated according to our proprietary technology. Our short-term plan is to introduce modified nanodiamonds with surface functional groups for conjugation to nucleic acid and protein molecules.

Detonation nanodiamonds are of great interest for biochemistry and molecular biology because of their unique physicochemical properties, such as a highly developed surface of particles (270–280 m2/g) with a large number of surface ionogenic (carboxyl, carbonyl, hydroxyl, ether) groups, hydrocarbon fragments, and metal microimpurities. Taken together, these factors allow one to treat nanodiamonds as new sorbents suitable for the separation, purification and immobilization of nucleic acids and proteins.

DNA molecules attached to diamond surfaces are accessible to enzymes, and can be modified in surface enzymatic reactions. Direct comparisons of DNA-modified  diamond surfaces with other commonly used surfaces, such as gold, silicon, and glassy carbon, showed that the modified diamond surfaces possessed excellent specificity and chemical stability under the conditions of repeated DNA hybridization/denaturation cycles (Lu, M. et al., Biopolymers, 2004).

 

 

References/Applications

  • Ligand exchange protein isolation and purification.

Physics of the Solid State 2004, 46 ( 4), 758–760. Translated from Fizika Tverdogo Tela,2004  46(4),  737–739.

Doklady Biochemistry, 2000, 373, 142–144. Translated from Doklady Akademii Nauk 2000, 373  (4) 547–549.

 

  • Boron-doped diamond electrodes.

Electroanalysis 2003, 15(3): 169-174.

J.Solid State Electrochem. 2001, 5: 88-93.

J.Solid State Electrochem. 2001, 5: 313-318

 

  • DNA immobilization, detection of SNP.

Biopolymers, Vol. 73, 606–613 (2004)

 

  • Modification of the nanodiamonds.

J. Surf Interface Anal 2001, 32, 3–9.

J. Nat Mater 2002, 1, 253–257.

Langmuir 2003, 19, 1938–1942.

 

 

             

 

 

Characteristics and Features

NanoD1

NanoD2

Colour

black

grey

Form

powder

powder

Grid parameters

0,3561±0.007nm

0,3561±0.007nm

Average size of crystalline grains

3.5 nm

3.5 nm

Estimated density

3.56 g/cm3

3.56 g/cm3

Bulk density

0.7 - 0.5 g/cm3

0.5 - 0.3 g/cm3

Surface

420 m2/g

300 m2/g

Content of the non-diamond carbon *

reduced

heightened

Water suspension formation without sonication

+

+

Capability for multiple drying of hydrosol and hydrosol formation by adding water to the dry powder

+

+

Water suspension stability

high

moderate

Minimal size of clusters in water suspensions

+

-

Aggregation at water suspension freezing and defrosting

-

+

Aggregation at boiling or autoclaving

-

+

Suspension stability in polar solvents

high

moderate

Colloidal stability in oils (no sediment formation during 2 months)

+

-

Possibility to get certain concentrations in water suspensions

+

+

Surface contamination of the particles *

reduced

reduced

Application as sorbent for column chromatography

+

-

Application for electrophoresis

+

-

*Compared to the raw material.

 

Ordering information

 NanoD1

Cat.#

Pack Size

ND-101

1 g

ND-105

5 g

ND-125

25 g

 NanoD2

Cat.#

Pack Size

ND-201

1 g

ND-205

5 g

ND-225

25 g

Both products are available in bulk quantities >100g. Please inquire price here. Hit Counter


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