Oligonucleotides Synthesis Service

15-150 nt-long, any scale and modifications

Oligonucleotides synthesis:

Quality: Reverse phase purification of oligonucleotides as standard procedure

Delivery: 3, 5 and 7 working days for unmodified, single-, and double- labelled oligonucleotides respectively.

Ordering via: email (sales@york-bio.com), fax (01904 567 621), mail, or online order form

Price starts from 30p/base (10 nmole scale).

Discounts: 10% off the price for >10 oligos/order.

Report: Complete documentation is enclosed.

Your Choice: Let us know if you would like to receive oligonucleotides dissolved in water or TE at working concentration.

Standard Oligonucleotides:

Synthesis Scale	Number of Bases	Yield (20-mer)	Purification	Price/base, (£)
10 nmole	15 - 35	2 OD	RPC*	0.40
40 nmole	15 - 80	4 OD	RPC*	0.50
40 nmole	80-150	2 OD	HPLC**	1.20
0.2 µmole	15 - 80	15 OD	RPC*	0.80
1.0 µmole	15 - 80	50 OD	Gel-filtration	1.80
10 µmole	15-50	400 OD	HPLC, £250	15.0
15 µmole	15-50	600 OD	HPLC, £350	18.0

Purification: *RPC (reverse phase chromatography) and gel-filtration are free of charge. HPLC** purification for 1 µmole and below - £20.

Prices on Labelled Oligonucleotides (15-80 bases), including HPLC purification:

Label or Modification	10 nmole	40 nmole	0.2 µmole	1.0 µmole	Unit
Standard DNA	£0.40	£0.50	£0.80	£1.80	base
Phosphorothioate	£5.80	£7.50	£8.50	£12.00	base
5’-Amino	£50	£60	£75	£85	oligo
3’-Amino	£50	£60	£75	£85	oligo
5’-Phosphorylation	£50	£60	£75	£85	oligo
3’-Phosphorylation	£40	£50	£80	£100	oligo
Biotin	£50	£60	£75	£85	oligo
FITC	£40	£60	£75	£85	oligo
TRITC	£40	£60	£75	£85	oligo
6-Fam	£60	£80	£95	£120	oligo
Tet	£60	£80	£95	£120	oligo
Hex	£60	£80	£95	£120	oligo
6-Rox	£80	£90	£110	£160	oligo
6-Joe	£80	£90	£110	£160	oligo
6-Tamra	£80	£90	£110	£160	oligo
Texas Red	£80	£90	£110	£160	oligo
Cy5	£80	£90	n/a	n/a	oligo
Cy3	£80	£90	n/a	n/a	oligo
DNP (Dinitrophenyl)	£60	£80	£95	£120	oligo
Dabsyl	£60	£80	£95	£120	oligo
Dual labelled oligonucleotides, including 2×HPLC: Possible dyes at 5’: 6-Fam, Hex, Tet Possible dyes at 3’: Tamra, Rox, Joe	£240	£300	£400	£800	oligo
Guaranteed yield	1.0 OD	2.0 OD	5.0 OD	15.0 OD	oligo

For oligos shorter than 15 or longer than 80 bases, please enquire.

Oligonucleotides order form:

Please provide your contact details (*- required fields):

*First Name

*Last Name

Title

* Phone

FAX

*E-mail

Please proceed to ordering unmodified or /and modified/labelled oligonucleotides. Prices, terms and conditions are in the table above.

NOTE: use following abbreviation when ordering oligonucleotides containing degenerate bases:

· A+G=R	· G+T=K	· A+T+C=H	· G+A+T=D	· Deoxyinosine =I
· C+T=Y	· G+C=S	· G+T+C=B	· A+C+T+G=N	· Deoxyuridine =U
· A+C=M	· A+T=W	· G+A+C=V

UNMODIFIED OLIGONUCLEOTIDES ORDER FORM:

OLIGO NAME

5'-SEQUENCE- 3'

SCALE
PURIFICATION

MODIFIED AND LABELLED OLIGONUCLEOTIDES ORDER FORM (HPLC purified only):

MODIFICATION/LABELLING

OLIGO NAME

5'-SEQUENCE- 3'

SCALE

5'-end

3'-end

internal

Notes or comments on oligonucleotides modification or labelling (use table above for options):

*Purchase Order #:

Account Name if known:

SHIPPING ADDRESS:

*Organization

*Department/Address

Address (cont.)

*City

*State/County

*Zip/Postal Code

*Country

* - required fields

BILLING address if different from above:

Organization

Street Address

Address (cont.)

City

State/County

Zip/Postal Code

Country

Phone

FAX

E-mail

VAT status:

Payable
Exempt (please fax or send the certificate)

DO NOT FORGET TO PRINT THIS FORM FOR YOUR RECORD!

			MODIFICATION/LABELLING
OLIGO NAME	5'-SEQUENCE- 3'	SCALE	5'-end	3'-end	internal

	SHIPPING ADDRESS:
*Organization
*Department/Address
Address (cont.)
*City
*State/County
*Zip/Postal Code
*Country

Organization
Street Address
Address (cont.)
City
State/County
Zip/Postal Code
Country
Phone
FAX
E-mail