Gene expression in mammalian cell lines

A convenient alternative for setting up mammalian cell facilities – get a comprehensive service from us.
We will achieve stable expression of the gene of your interest in mammalian cells.

Customer provides:

• Mammalian vector with the gene (cDNA) to be expressed. We accept plasmid and retroviral vectors
•  Sequence of the gene and map of the construct for transfection
• Cell line or information about the cell line to be transfected.

Our service includes:

• Transfection of the cells. In case of a retroviral vector, virus production and cell infection
• Antibiotic selection and generation of stable transfected (infected) cell clones. At least 10 independent clones will be selected and grown
• Quantitative assay of the gene (cDNA) expression level in each transfected clone by RNA isolation followed by Northern hybridisation and/or RT-PCR
• Selection of the best expressing clone
• Cell freezing and depositing
• Duration: 3-6 months (depending on the cell growth rate), allow 1month in addition if the cell line is not available in our collections

Customer receives:

• Detailed report on experiments and data obtained.
• Two vials of transfected cells (the best expressing clone)
• We will deposit the transfected cells in our collection as a precaution against accidental loss of the clone.

Price guide:

• Price per transfection and selection of at least 10 clones: £4500.