DNA Amplification

        DNA Polymerases (individual, mixes, heat-activated),

        Ready-To-Go Reaction Mixes for Your DNA Work

 

        Thermophilic DNA Polymerases

Name Features Pack Size

Conc.

 Cat. # Price Heat-activated version Ready-to-go Mix
YB-TAQ Wide-spread enzyme for broad range applications (up to 5 kb) YB-TaqMix 500 u

5 u/µl

 A1002 YB-TaqH £35
2,500 u

5 u/µl

 A2002 £140
10,000 u

5 u/µl

 A2001 £450
 YB-TAQ+ Same as YB-Taq, but with extended life-time at elevated temperatures (up to 8 kb) 100 u

5 u/µl

 A1003 £50 YB-Taq+H YB-TaqMix+
500 u

5 u/µl

 A1004 £120
VerizymeTM Proof-Reading DNA Polymerase for high fidelity amplification and subsequent cloning (up to 6 kb) 100 u

5 u/µl

 A1005 £100   YB-VeriMix
500 u

5 u/µl

 A1006 £250
YB-GCPol Mix of DNA Polymerases operating with GC-, or AT-rich DNA templates (up to 12 kb) 100 u

5 u/µl

 A1007 £100   YB-GCMix
500 u

5 u/µl

 A1008 £250
YB-LDPol Mix of DNA Polymerases for long-distance applications (up to 19 kb) 100 u

5 u/µl

 A1009 £100   YB-LDMix
500 u

5 u/µl

 A1010 £250
YB-SeqTaq Polymerase ideally suited for DNA sequencing or labelling (up to 5 kb) 100 u

5 u/µl

 A1013 £100 YB-SeqTaqH  
500 u

5 u/µl

 A1014 £250

 

Hot-Start DNA Polymerases.

Complete activation can be achieved in 15 seconds at >90 ºC.

Name Features

Pack Size

Conc.

Cat. #

Price
YB-TaqH Hot-Start form of YP-Taq

500 u

5 u/µl

A1101

£100
YB-Taq+H Hot-Start form of YP-Taq+

500 u

5 u/µl

A1103

£150
YB-SeqTaqH Hot-Start form of YP-SeqTaq

500 u

5 u/µl

A1113

£180

 

 

2x Ready-to-go Reaction Mix contains all necessary components of reaction mix at optimised concentrations (buffer, dNTPs, enzyme, additives, Mg2+). Available with additional vial of 10 mM Mg2+ should extra Mg be required for further optimisation.

Final concentration of Mg2+ in 1x Mix – 1.5 mM.

Mixes do not contain template DNA and Oligonucleotides.

Simply take 25 µl of 2xMix, add template DNA, Oligonucleotides, Mg (if needed), and molecular biology grade water up to 50 µl. Run reaction program.

In cases - short fragments, or when accuracy is of priority – dilution of Mixes in more than 2 times is recommended.

Name

Features

Polymerase conc., u/µl

Mg conc.,mM

Pack size, reactions

 Cat. # Price

2x

1x

2x

1x
YB-TaqMix YB-Taq

0.1

0.05

3

1.5

200

A1201

£180
YB-TaqMix+ YB-Taq+

0.1

0.05

3

1.5

200

A1203

£180
YB-VeriMix VerizymeTM

0.1

0.05

3

1.5

200

A1205

£220
YB-GCMix YB-GCPol

0.1

0.05

3

1.5

200

A1207

£260
YB-LDMix YB-LDPol

0.08

0.04

3

1.5

200

A1209

£240

 

Characteristics of Yorkshire BioScience Thermophilic DNA Polymerases
Characteristics YB-Taq YB-Taq+

Verizyme

 YB-GCPol YB-LDPol YB-SeqTaq
3’-5’ proofreading, Y/N

N

N

Y

Y

Y

N
5’-3’ exonuclease, Y/N

Y

Y

N

Y

Y

Y
Half-life at 95 ºC

1 hour

1 hour

8 hours

2 hours

2 hours

1 hour
Units to add per 100 µl of primer extension reaction mix

2-10

2-10

1-3

2-4

2-4

2-10
Allow time for extension

20 sec/kb

20 sec/kb

50 sec/kb

40 sec/kb

30sec/kb

20 sec/kb
Error Rate, x10-6 base insertions

200-400

200-400

10-60

30-80

30-80

100-300
Optimum and maximum of Primer Extension

0.05-3kb; 5kb

0.05-5kb; 8kb

0.05-4kb; 6kb

0.1-8kb; 12kb

1-10kb; 19kb

0.05-2kb; 5kb
3'-DNA Ends dA-tailed dA-tailed blunt mixed mixed dA-tailed
Specific Applications YB-Taq YB-Taq+

Verizyme

 YB-GCPol YB-LDPol YB-SeqTaq
Broad range

++

+++

++

+

+

++
Long Distance (>5 kb)

-

+

-

++

+++

-
Short Distance (<500 bp)

+++

++

++

++

+

+++
Difficult templates (GC-,or AT- rich)

+

++

++

+++

++

+
DNA Labelling

+

+

-

-

+

+++
High Fidelity (low error rate)

+

+

+++

++

++

+
Heat activated version available, Y/N

Y

Y

N

N

N

Y
Ready-to-go Mix available,Y/N

Y

Y

Y

Y

Y

N