DNA Molecular Weight Markers

DNA MW 0.1-14kb (Lambda - BstE II Digest)

Description: 14 fragments ranged from 117bp to 14,140bp suitable for use as quantitative molecular weight marker for agarose gel electrophoresis.

Concentration: 200 µg/µl

Recommended volume to load - 5 µl, equivalent to 1 µl of DNA

Storage: 2 months at +4 °C, 1 year at -20 °C.

Shipment at ambient temperature.

5 µl (1 µg) of DNA MW 0.1-14kb, 1% agarose, stained with ethidium bromide:

	Marker Composition, 5µl: Size, bp Mass, ng 14,140 290 7,242 150 6,369 130 4,822 100 4,324 90 3,675 75 2,323 50 1,929 40 1,371 30 1,264 25 702 15 224* 5 117* 2 *Bands corresponding to 224 bp and 117 bp are minor and may not be visible. NOTE: Fragment 14,140 bp is composed of fragments 8,454 bp and 5,686 bp with cohesive ends that may be  separated by heating at 60 °C for 3 minutes. Higher temperature may cause DNA denaturation.