|
Primer Name |
Primer Sequence |
Tm |
For Vector |
|
3AOX1 |
GCAAATGGCATTCTGACATCC |
60.85 |
pPICZa |
|
5AOX1 |
GACTGGTTCCAATTGACAAGC |
60.85 |
pPICZa |
|
LIB M13F |
GTAAAACGACGGCCAGT |
56.31 |
pLIB-DNR |
|
LIB-M13R |
AAACAGCTATGACCATG |
51.49 |
pLIB-DNR |
|
Mal E |
GGTCGTCAGACTGTCGATGAAGCC |
68.81 |
pMAL-c2X |
|
M13F -
24mer |
CGCCAGGGTTTTCCCAGTCACGAC |
70.52 |
M13,
pBluescript, pcr script, pGem32, pGem32f, pGemT, Teasy, puc19, puc18,
pGem2 vectors |
|
M13R -
22mer |
TCACACAGGAAACAGCTATGAC |
61.26 |
M13,
pBluescript, pcr script, pGem32, pGem32f, pGemT, Teasy, puc19, puc18,
pGem2 vectors |
|
OpIE2F |
CGCAACGATCTGGTAAACAC |
60.40 |
pMIB-V5-His |
|
OpIE2R |
GACAATACAAACTAAGATTTAGTCAG |
59.44 |
pMIB-V5-His |
|
pETBlueT7_up |
TCATAACGTCCCGCGAAA |
57.06 |
pETBlue-1_Novagen |
|
pETBlue_down |
GTTAAATTGCTAACGCAGTC |
56.30 |
pETBlue-1_Novagen |
|
pETBlue_up |
TCACGACGTTGTAAAACGAC |
58.35 |
pETBlue-1_Novagen |
|
pGEX forward |
GGGCTGGCAAGCCACGTTTGGTG |
70.55 |
pGEX |
|
pGEX reverse |
CCGGGAGCTGCATGTGTCAGAGG |
70.55 |
pGEX |
|
pIB FP |
GGATGTGCTGCAAGGCGATTAAGTTGG |
68.93 |
pIindigoBAC-5 |
|
pIB RP |
CTCGTATGTTGTGTGGAATTGTGAGC |
65.74 |
pIndigoBAC-5 |
|
polyT20N |
TTTTTTTTTTTTTTTTTTTTN |
39.90 |
sequencing after polyA tails |
|
SP6 |
TATTTAGGTGACACTATAG |
51.27 |
pGem32, pGem32f, pGemT, Teasy, pBieloBAC II, pGem2 vectors, lambdaGem,
pGemex |
|
T3 -
17mer |
ATTAACCCTCACTAAAG |
49.07 |
supercos I |
|
T3 -
20mer |
ATTAACCCTCACTAAAGGGA |
56.30 |
M13,
pBluescript, pcr script, pGem2 vectors, pGemex |
|
T7 -
17mer |
AATACGACTCACTATAG |
49.07 |
supercos I |
|
T7 -
20mer |
TAATACGACTCACTATAGGG |
56.30 |
M13,
pBluescript, pcr script, pGem32, pGem32f, pGemT, Teasy, pBieloBAC II,
pGem2 vectors, pIndigoBAC-5 |
|
T7Term |
GCTAGTTATTGCTCAGCGG |
59.90 |
pCRT7-TOPO |
|
M13F
17-mer |
GTAAAACGACGGCCAGT |
56.31 |
pLIB-DNR |
