Random DNA mutagenesis

We can introduce random mutations to the gene supplied by a customer.
Mutated DNA will be then cloned into a vector of choice to obtain a library of the mutated DNA

• Mutation frequency can be set to a desired value within 1-20 mutations per kb
• Number of clones 10⁶-10⁸
• Mutations can be restricted to a desired region of a gene
   

Customer provides:
Vector/plasmid containing DNA to be mutated
Map of that plasmid and sequence of the target DNA
Instructions on desired mutation frequency and number of clones
 

Our service includes:
DNA mutagenesis specified
Cloning of the mutations library into a vector of choice

 Duration:
10⁶ clones: 3- 4 weeks
10⁸ clones: 7-8 weeks
 

Customer receives:
Report with mutation library characteristics and sequence data for 10 randomly selected clones
E. coli stock containing a mixture of clones with unique random mutations mutations
 

Price guide:
10⁶ clones: £2900
10⁸ clones: £3700