Site Directed DNA Mutagenesis

Site Directed DNA Mutagenesis

Study the structure and function of the protein you are working on with our DNA mutagenesis

Deletions, insertions or substitutions
Mutations in plasmids of size up to 15 kb

Customer provides:

Vector/plasmid containing DNA to be mutated
Map of that plasmid and sequence of the target DNA
Instructions on mutagenesis: list of bases for insertions, deletions and substitutions

Our service includes:
Design and synthesis of oligonucleotides
DNA mutagenesis specified
Sequencing of the target DNA in both directions if necessary

Duration: 2-5 weeks depending on the length of a plasmid

Customer receives:
Sequencing data
1.0 µg of verified DNA purified from a single clone

Price guide:

Number of single mutations per plasmid	Price/single mutation
Number of single mutations per plasmid	Plasmid size up to 6 kb	Plasmid size 6-15 kb
1	£500	£650
2-5	£400	£500
>5	£350	£400