High Throughput Open Reading Frames Cloning
and Heterologous Expression
We provide cloning and optimisation of conditions
(temperature, IPTG concentration,
duration, protein solubility) for heterologous expression of Open Reading Frames
(ORF)
from various organisms in E. coli.
Customer provides:
- Genomic DNA
- Complete sequence of each ORF that needs to be
assayed
Our service includes:
- Primer design and synthesis, cloning and
sequencing of ORFs of interest
- Trial expression of ORF in E.coli, protein
solubility assay
- Optimisation of conditions for heterologous
expression according to the customer’s requirements:
Temperature
Inducer (e.g. IPTG) concentration
Duration of expression
- PAGE of samples obtained during optimisation.
Customer receives:
- Report on ORF cloning and DNA sequence data
analysed with standard software
- Data on expression optimisation with PAGEs on
protein yield and solubility
- Optional: optimisation of buffer composition for
protein extraction
- ORF DNA in cloning and/or in expression vector.
Prices start from:
£100 per clone of individual ORF with confirmed DNA
sequence,
£60 per trial expression,
£140 per optimisation of individual ORF expression.
The prices are based on a minimum of 50 ORFs per order.
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