Description: 14 fragments ranged from 117bp to 14,140bp suitable for use as
quantitative molecular weight marker for agarose gel electrophoresis.
Concentration: 200
µg/µl
Recommended volume to load - 5 µl, equivalent to 1 µl of
DNA
Storage: 2 months at +4 °C, 1 year at -20 °C.
Shipment at ambient
temperature.
5 µl (1 µg) of
DNA MW 0.1-14kb, 1% agarose, stained with ethidium bromide:
Marker Composition, 5µl:
Size, bp
Mass, ng
14,140
290
7,242
150
6,369
130
4,822
100
4,324
90
3,675
75
2,323
50
1,929
40
1,371
30
1,264
25
702
15
224*
5
117*
2
*Bands corresponding
to 224 bp and 117 bp are minor and may not be visible.
NOTE: Fragment
14,140 bp is composed of fragments 8,454 bp and 5,686 bp with
cohesive ends that may be separated by heating at 60
°C for 3 minutes. Higher temperature may cause
DNA denaturation.