Monoclonal Antibody Production
Price: £4000
Duration: 4 months
We offer
monoclonal antibody (MAb) development in mice, with guaranteed
success - three positive and stable clones against the antigen
used in an ELISA. We have experience in raising antibodies
against substances of low immunogenicity. All features of the
antigen will be discussed before the start of a project.
Customer will be regularly updated on the project’s progress.
We can predict
success or failure of the project after mice immunisation. In
case of a failure we provide complete report on the experiments,
explain the reason for the failure and charge only for the first
phase of the project – 10% of total cost.
Customer
provides:
-
Approximately 2-3 mg of protein antigen or 2 mg of
conjugated peptide and 0.5 mg of free peptide for
ELISA.
- Information
on the antigen nature and properties.
Customer
receives:
- 3 hybridoma
clones, cells aliquots in 3 cryo vials per clone.
- A
certificate and protocol of the project.
Standard
Service:
Phase I (9
weeks)
-
Immunisation and boosts for 5 Balb/c mice with standard
immunisation protocol.
- Titer
assay (ELISA) prior to selection of donor mice.
Phase II (4
weeks)
-
Hybridoma fusion using splenocytes or lymph node cells and
SP2/0 myeloma cells.
-
Screening of up to 2,000 hybridoma clones for positives with
ELISA. Up to 10 highly positive clones will be subcloned,
expanded, and 5 ml of hybridoma culture medium will be
provided to customer for evaluation.
Phase III (2
weeks)
-
Customer selects 3 clones that will be expanded for
cryopreservation and sent to the customer.
- We will
keep those 3 selected hybridoma cells for next 6 months for
free.
Additional
Services:
- Large
scale antibody production in ascitic fluid or in stationary
cell culture.
- Peptide
synthesis and conjugation to a carrier.
-
Additional clones for phases II and III.
- Protein
A or protein G affinity purification.
-
Antibody biotinylation.
-
Evaluation of suitable screening techniques other than
ELISA.