PCR Buffers

Choosing the right buffer for a particular DNA polymerase and specific application plays a key role in PCR optimisation. Poor performance of enzymes, low or no yield are consequences of employing the wrong buffer, in most cases. We offer both individual and sets of 10 × PCR buffers which have emerged during history of PCR for screening purposes. Buffers are prepared using DNase- and RNase-free water. The pH was measured at 25 ºC. All buffers were assayed in reactions with a number of commercially available DNA polymerases. Detergent-free buffers suitable for DHPLC are highlighted.

All buffers can be supplemented with MgCl₂, MgSO₄, or Mg-Acetate upon request.

Please contact us if you need any advice on optimum buffer composition for a PCR procedure you experience difficulties with.

Name	Composition, 10 ×	pH	Pack size	Cat #	Price
A	375mM Tris-Cl, 200mM (NH₄)₂SO₄, 0.1% Tween-20	8.8	5 × 1.2 ml	S1102	£20
B	670mM Tris-Cl, 150mM (NH₄)₂SO₄, 0.1% Tween-20	8.8	5 × 1.2 ml	S1104	£20
C	1.0M Tris-Cl, 200mM (NH₄)₂SO₄, 0.1% Tween-20	8.4	5 × 1.2 ml	S1106	£20
D	600mM Tris-Cl, 170mM (NH₄)₂SO₄, 0.1% Tween-20	9.0	5 × 1.2 ml	S1108	£20
E	500mM Tris-Cl, 200mM (NH₄)₂SO₄	8.4	5 × 1.2 ml	S1110	£20
F	500mM Tricin-KOH, 170mM (NH₄)₂SO₄, 0.1% Tween-20	8.7	5 × 1.2 ml	S1112	£20
G	500mM Tricin-KOH, 150mM (NH₄)₂SO₄	8.7	5 × 1.2 ml	S1114	£20
H	500mM Tris-Cl, 500mM KCl	8.4	5 × 1.2 ml	S1116	£20
I	400mM Tris-Cl, 500mM NaCl	7.5	5 × 1.2 ml	S1118	£20
J	500mM Tricin-KOH, 450mM KAcetate, 5% DMSO, 40% glycerol	8.7	5 × 1.2 ml	S1120	£20
K	500mM Tris-Cl	9.5	5 × 1.2 ml	S1122	£20
L	100mM Tris-Cl, 50mM DTT	7.5	5 × 1.2 ml	S1124	£20
M	100mM Tris-Cl, 100mM KCl	8.3	5 × 1.2 ml	S1126	£20
N	100mM Tris-Cl, 500mM KCl, 1% Triton X-100	8.9	5 × 1.2 ml	S1128	£20
O	100mM Tris-Cl, 500mM KCl	8.0	5 × 1.2 ml	S1130	£20
P	400mM Tris-Cl, 650mM K-Glutamate, 1% Triton X-100	8.7	5 × 1.2 ml	S1132	£20
Q	400mM Tris-Cl, 650mM K-Acetate, 1% Triton X-100	8.7	5 × 1.2 ml	S1134	£20
R	600mM Tris-Cl, 60mM (NH₄)₂SO₄, 100mM KCl	8.3	5 × 1.2 ml	S1136	£20
S	500mM Tris-Cl, 50mM (NH₄)₂SO₄, 100mM KCl	9.0	5 × 1.2 ml	S1138	£20
T	200mM Tris-Cl, 100mM (NH₄)₂SO₄, 100mM KCl, 1% Triton X-100	8.9	5 × 1.2 ml	S1140	£20
U	1M Tris-Cl, 100mM (NH₄)₂SO₄, 100mM KCl, 1% Triton X-100	8.7	5 × 1.2 ml	S1142	£20
V	200mM Tris-Cl, 100mM (NH₄)₂SO₄, 100mM KCl, 1% Triton X-100, 1mg/ml BSA	8.7	5 × 1.2 ml	S1144	£20
W	100mM Tris-Cl, 500mM KCl, 1% Triton X-100	8.3	5 × 1.2 ml	S1146	£20

10 × PCR Buffers Set 1 (no detergents)
Description:	Set of 10 × PCR buffers E, G, H, I, J, K, L, M, O, R, S
Content:	11 × 1.2 ml of each buffer 1 × 1.2 ml of 50mM MgCl₂ 1 × 1.2 ml of 50mM MgSO₄ 1 × 1.2 ml of 50mM Mg acetate Protocol
Storage:	24 months at -20 ºC in a constant temperature freezer
Catalogue #	S2101
Price	£60

10 × PCR Buffers Set 2 (with detergents)
Description:	Set of 10 × PCR buffers A, B, C, D, F, N, P, Q, T,U, V, W
Content:	12 × 1.2 ml of each buffer 1 × 1.2 ml of 50mM MgCl₂ 1 × 1.2 ml of 50mM MgSO₄ 1 × 1.2 ml of 50mM Mg acetate Protocol
Storage:	24 months at -20 ºC in a constant temperature freezer
Catalogue #	S2102
Price	£70

10 × PCR Buffers Set A-W
Description:	Set of 10 × PCR buffers A to W
Content:	23 × 1.2 ml of each buffer 1 × 1.2 ml of 50mM MgCl₂ 1 × 1.2 ml of 50mM MgSO₄ 1 × 1.2 ml of 50mM Mg acetate Protocol
Storage:	24 months at -20 ºC in a constant temperature freezer
Catalogue #	S2101
Price	£110

2 × DNA Softner
Description: Universal additive to any PCR mix. Proprietary optimised composition of several additives. Helps in all difficult cases. Improves specificity, sensitivity and yield of reaction as well as performance of polymerases. Action is similar to “melting” of DNA regions with complex secondary structure and making DNA more accessible to polymerase. Tested at up to 76% of GC-, and 68% of AT-rich sequences.
Applications: PCR on GC- or AT-rich templates including DNA sequencing, cloning and detection.
Shipping Conditions: Any temperature equal or below ambient
Storage Conditions: +4 ºC for short time (up to 2 weeks), -20 ºC for months
Note: If precipitate – heat at 65 ºC for 5 min and vortex until crystals disappear.
	Cat.#	Pack Size	Price
	S1150	Sample 0.25 ml	Free of charge
	S1151	1.2 ml	£30
	S1152	5 × 1.2 ml	£80